Fluorometric Quantitation of dsDNA using PicoGreen® Using SynergyTM Neo2 Multi-Mode Reader to Quantify DNA in Solution

Many techniques of cellular and molecular biology require the ability to quantify dsDNA in large numbers of samples at sensitivities that only require a small amount of the total sample. Isolation of plasmids from bacterial cultures, genomic DNA from mammalian cells, cDNA synthesis for library production, and quantitation of PCR products for diagnostic purposes all require the direct quantitation of dsDNA. Also, many biochemical studies that involve the growth kinetics of cell cultures or cell cycle studies require normalization by DNA content.